Carsten Burkhardt's Web Project Paeonia - The Peony Library

ano_finnland7 index 0602 boreas2

Paeonia-Literatur 2003

Chen JW, Li X, Yuan ZR. [Rapid identification of Cortex moutan radicis and similar aromatic Chinese herbs by headspace gas chromatography] [Article in Chinese] Zhong Xi Yi Jie He Xue Bao. 2003 Sep;1(3):215-7. [OBJECTIVE: To establish the method for rapid identification of the quality of Cortex moutan radicis and similar aromatic Chinese herbs by headspace gas chromatography (HSGC). METHODS: The constituents of headspace gas from Chinese herbs, such as Cortex moutan radicis,Radix cynanchi paniculati and root-bark of Paeonia lactiflora. were analyzed by HSGC. RESULTS: The characteristic finger-print headspace chromatograms of these three drugs were different from one another. Cortex moutan radicis stored for 4 years under common conditions contained only traces of paeonol. CONCLUSION: HSGC was simple, quick and accurate in identifying and controlling the quality of aromatic Chinese herbs.] PMID: 15339567 [PubMed - indexed for MEDLINE] Institute of Medicinal Fungi & Bio-Technology of Traditional Chinese Medicine, College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China.

He JX, Akao T, Tani T. Restorative effect of repetitive administration of Shaoyao-Gancao-tang on bioavailability of paeoniflorin reduced by antibacterial synthetic drugs treatment in rats. Biol Pharm Bull. 2003 Nov;26(11):1585-90. [Abstract: Paeoniflorin (PF) is an active glucoside in Shaoyao (peony root), and is transformed into an antispasmodic metabolite, paeonimetabolin-I (PM-I), by intestinal bacteria in the gut after oral administration of Shaoyao or Shaoyao-Gancao-tang (SGT, Shakuyaku-Kanzo-To in Japanese). SGT is a pain-relieving traditional Chinese formulation (Kampo-medicine in Japanese) and is often used together with antibacterial synthetic drugs, such as amoxicillin and metronidazole (AMPC-MET), in peptic ulcer therapy. Since the bioavailability of PF in SGT has been reported to be significantly reduced by co-administered antibacterial drugs, we investigated how to minimize this reducing effect of antibacterial treatment in the present study. We found that repetitive administration of SGT starting 24 h after AMPC-MET treatment rapidly restored the plasma PM-I concentration from SGT reduced by AMPC-MET, due to its restorative effect on the decreased PF-metabolizing activity of intestinal bacteria in rat feces. The present findings suggest that it may be clinically useful to administer SGT repetitively, starting 1 or 2 d after treatment with a mixture of AMPC-MET during their combination therapy, to accelerate the recovery of the reduced bioavailability of PF in SGT. Similar administration regimens may also be useful in other combination therapies involving traditional Chinese formulations and antibacterial synthetic drugs to ensure the efficacy of the bioactive glycosides in the formulations.] Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama, Japan. Click here to read

Hong D Y & Zhou S L, 2003: Paeonia (Paeoniaceae) in the Caucasus. Bot. J. Linn. Soc. 143: 135—150. [Abstract: The taxonomy of the genus Paeonia in the Caucasus has been controversial, with recognized species varying in number from one to 13. The taxonomic history of Paeonia in this area is reviewed (including an analysis of the characters used by previous authors) based on extensive field observations, population sampling and critical examination of a large number of herbarium specimens. The results show that Paeonia may be divided into three groups. The P. intermedia group is known from only a single population. In the P. tenuifolia group, all the characters used for distinguishing the three previously recognized species were found to be polymorphic. In the P. daurica group, petal colour, shape and size of leaflets, and indumentum of leaflets and carpels were used to distinguish nine species, but these characters were found to be polymorphic or continuous in variation, and thus can only be used for infraspecific classification. Thus, three species are recognized: P. intermedia, P. tenuifolia and P. daurica. The last species is further divided into five subspecies: sspp. coriifolia, wittmanniana, mlokosewitschii, macrophylla and tomentosa stat. nov. © 2003 The Linnean Society of London, Botanical Journal of the Linnean Society, 2003, 143, 135-150.] (link to a local copy .pdf-file)

Hong H, Wang QM, Zhao ZP, Liu GQ, Shen YS, Chen GL. Studies on antidiabetic effects of cortex Moutan polysaccharide-2b in type 2 diabetes mellitus rats. Yao Xue Xue Bao. 2003 Apr;38(4):255-9. [Abstract: To study the antidiabetic effects of cortex Moutan polysaccharide-2b (CMP-2b) in type 2 diabetes mellitus (T2DM) rats. METHODS: The T2DM model rats were induced by a single intravenous injection of low dose streptozotocin (STZ) and intake of high sucrose-fat diet. CMP-2b was given to T2DM rats daily through gavage for 4-5 weeks. The body weight, water and food intake, fasting blood glucose (FBG), glucose tolerance, plasma lipids, serum insulin, and insulin receptor (Ins R) were determined. RESULTS: Oral administration of CMP-2b significantly decreased water and food intake, FBG, total cholesterol (Tch), and triglyceride (TG), improved the impaired glucose tolerance (IGT), and remarkably raised the number of low affinity InsR and insulin sensitivity index (ISI) in T2DM rats. CONCLUSION: CMP-2b may be useful for treating T2DM and its complications. PMID: 12889122 [PubMed - indexed for MEDLINE] ( Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.)

Hsiu SL, Lin YT, Wen KC, Hou YC, Chao PD. A deglucosylated metabolite of paeoniflorin of the root of Paeonia lactiflora and its pharmacokinetics in rats. Planta Med. 2003 Dec;69(12):1113-8. [Abstract: Paeoniflorin is a bioactive monoterpene glucoside in Paeoniae Radix (PR), the roots of Paeonia lactiflora (Ranunculaceae). By oral administration to rats with the decoction of PR, the metabolism and pharmacokinetics of paeoniflorin were investigated in this study. A deglucosylated metabolite of paeoniflorin, paeoniflorgenin (PG), in serum was identified based on HPLC/MS and NMR spectral data. HPLC/UV methods were developed for determining PG in serum and feces suspension. A non-compartment model was used for the calculation of pharmacokinetic parameters. Moreover, the metabolism of paeoniflorin by various types of feces was investigated as well. The paeoniflorin levels in serum were below the detection limit throughout the study. The C (max), t (max), and AUC (0-t) of PG were 8.0 microg/mL, 10 min and 487.0 microg min/mL, respectively. Paeoniflorin was found to be hydrolyzed into PG through incubation with feces of rabbit, rat, pig or human. Similar profiles of PG were shown for various types of feces, except for rabbit. In conclusion, paeoniflorin was not absorbed per se, whereas its aglycone paeoniflorgenin was absorbable and circulating in the bloodstream. Rat and pig are appropriate models for investigating the metabolism and pharmacokinetics of paeoniflorin.] Department of Pharmacy, China Medical University, Taichung, ROC. Click here to read

Huang L, Shi P, Wang X. [The effect of the extract from Radix Paeoniae alba on IgA Glomerulonephritis in mice] [Article in Chinese] Zhong Yao Cai. 2003 Feb;26(2):109-11. [OBJECTIVE: To observe the effect of the extract from Radix Paeoniae Alba on IgA glomerulonephritis in mice. METHODS: IgA glomerulonephritis was induced by injection of dextran and sephadex-150. After administrating the extract, the contents of urinary protein, BUN and Cr in serum were determined. RESULTS: The extract could inhibit the decline of mouse weight, and decrease urinary protein content and BUN content in serum. While, the extract had no effect on Cr in serum. CONCLUSION: The extract from Radix Paeoniae Alba had therapeutical effect on IgA glomerulonephritis.] No. 451 Hospital of PLA, Xi'an 710054.

Kamenetsky R, Barzilay A, Erez A, Halevy AH (2003) Temperature requirements for floral development of herbaceous peony cv. 'Sarah Bernhardt'. Scientia Horticulturae 97, 309-320.

Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. Screening of medicinal plant extracts for antioxidant activity. Life Sci. 2003 May 30;73(2):167-79. [Abstract: The methanol extracts of nine medicinal plants traditionally used in Chinese medicine were screened for antioxidant activity versus resveratrol, which has been shown to protect cells from oxidative damage [Toxicol. Lett. 102 (1998) 5]. Most of the plant extracts used in this study inhibited the H(2)O(2)-induced apoptosis of Chinese hamster lung fibroblast (V79-4) cells. The extracts of Areca catechu var. dulcissima, Paeonia suffruticosa, Alpinia officinarum, Glycyrrhiza uralensis and Cinnamomun cassia strongly enhanced viability against H(2)O(2)-induced oxidative damage in V79-4 cells. Relatively high levels of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were detected in extracts of Areca catechu var. dulcissima, Paeonia suffruticosa and Cinnamomun cassia (IC(50) < 6.0 microg/ml). The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were dose-dependently enhanced in V79-4 cells treated with most of the plant extracts. The extracts of Areca catechu var. dulcissima showed higher antioxidant activity than resveratrol in all experiments. These results suggest that the plant extracts prevent oxidative damage in normal cells probably because of their antioxidant characteristics.] Department of Biochemistry, College of Dentistry, Kyung Hee University, 1 Hoegi-Dong, Dongdaemoon-Ku, Seoul 130-701, South Korea. Click here to read

Liang QD, Li WH, Wang HX, Wang SQ. [Saccharide analysis of si-wu-tang by automated multiple development and off-line coupling thin layer chromatography electrospray ionization mass spectrometry techniques] [Article in Chinese] Zhongguo Zhong Yao Za Zhi. 2003 Jul;28(7):632-5.[OBJECTIVE: To make qualitative analysis on saccharide spots in thin layer chromatography (TLC) chromatogram of SI-WU-TANG extract C, which possesses blood-enrichment activity. METHOD: TLC chromatogram of SI-WU-TANG extract C was obtained by using Automated Multiple Development (AMD) method. 4 major spots in the chromatogram were analyzed by off-line coupling TLC electrospray ionization mass spectrometry (ESI-MS) technique. Moreover, composition of monosaccharides in the fraction was analyzed by AMD technique. RESULT: Main constituents of substances from the 4 spots were monosaccharide, disaccharide, trisaccharide and tetrasaccharide respectively. Monosaccharide was mainly composed of fructose and glucose. CONCLUSION: Off-line coupling TLC ESI-MS can simply and rapidly provide qualitative examination of saccharide spots in TLC chromatogram of Traditional Chinese Medicine. AMD method can make good separation of 8 frequently-observed monosaccharides in a regular 10 cm silica gel plate, the process of which was automated, AMD and off-line coupling TLC ESI-MS techniques show good value in saccharides analysis.] Institue of Radiation Medicine, Acadamy of Military Medical Sciences, Beijing 100850, China.

Li YC, Sun YF, Feng ZJ. [Experimental study on antagonizing liver fibrosis of radix Paeoniae rubra] [Article in Chinese] Zhongguo Zhong Xi Yi Jie He Za Zhi. 2003 Oct;23(10):767-8.

Luo H, Li X, Xiao F, Gu F. [Study on pharmacokinetics of ferulic acid and paeoniflorin in Angelic-Paeonia root powder] [Article in Chinese] Zhong Yao Cai. 2003 Mar;26(3):189-92. [ OBJECTIVE: To study pharmacokinetics of ferulic acid and paeoniflorin that are two chief active compounds in Angelic-Paeonia Root Powder (APRP). METHODS: After the mice were administered by APRP through ig, HPLC was used to determine ferulic acid and paeoniflorin concentration in serum at different times. 3P87 procedure was used to process concentration-time data. RESULTS: Ferulic acid and paeoniflorin were detected from serum in the mice. The pharmacokinetics parameters were: Ferulic acid: Ke = 0.330 +/- 0.085 1/h, Ka = 0.555 +/- 0.133 1/h, t1/2(ka) = 1.249 +/- 0.365 h, t1/2(ke) = 2.101 +/- 0.665 h, Tpeak = 2.606 +/- 0.586 h, Cmax = 6.372 +/- 1.510 mg.L-1, AUC = 41.399 +/- 11.763 mg.h-1.L-1; Paeoniflorin: Ke = 0.511 +/- 0.117 1/h, Ka = 0.656 +/- 0.121 1/h, t1/2(Ka) = 1.056 +/- 0.263 h, t1/2(Ke) = 1.356 +/- 0.281 h, Tpeak = 2.062 +/- 0.396 h, Cmax = 3.401 +/- 0.879 mg.L-1, AUC = 16.047 +/- 3.767 mg.L-1. The present research provided valuable data for rational clinical practice of APRP.] College of Pharmacy, Drug Research Center, College of Pharmacy, Jinan University, Guangzhou 510632.

Mendez M, Traveset A. Sexual allocation in single-flowered hermaphroditic individuals in relation to plant and flower size. Oecologia. 2003 Sep;137(1):69-75. Epub 2003 Jul 3. [Abstract: Gender expression in hermaphroditic plant species usually departs from strict equisexuality. Study of those departures can aid understanding of non hermaphroditic breeding systems and prevalence of hermaphroditism within angiosperms. Plant size is one of the most studied factors in relation to gender modification. We studied variation in gender expression in the hermaphroditic, mostly single-flowered Paeonia cambessedesii. We separately studied gender modification with increasing plant and flower size using a variety of currencies: number of ovules and stamens, dry mass, N and P. Flower size and number of floral structures (petals, stamens, carpels, and ovules) increased with plant size. Number of ovules increased more rapidly with increasing plant size than number of stamens, indicating a bias towards femaleness with increasing plant size. A similar pattern was found when regressing number of stamens and number of seeds against plant size. Number of floral structures increased with increasing flower mass, but no significant difference was found between stamens and ovules in their rate of increase. Thus, gender modification at plant level was not consistent with patterns at flower level. No differential allocation to stamens vs gynoecium, or sexual structures vs petals was found when using dry mass, N or P as currencies. However, a disproportionate increase in female allocation was found when number of structures was utilised as currency. Study of size-dependent gender expression will benefit from contrast of results obtained using several analysis levels and allocation currencies] Department of Plant Ecology-Evolutionary Biology Centre, Uppsala University, Villavagen 14, 752 36 Uppsala, Sweden. Click here to read

Ne'eman G. (Department of Biology, Faculty of Science and Science Education, University of Haifa-Oranim, Tivon 36006, Israel) To be or not to be-the effect of nature conservation management on flowering of Paeonia mascula (L.) Miller in Israel Biological Conservation, January 2003, vol. 109, no. 1, pp. 103-109(7) Publisher: Elsevier Science DOI: 10.1016/S0006-3207(02)00143-X

Nishida S, Kikuichi S, Yoshioka S, Tsubaki M, Fujii Y, Matsuda H, Kubo M, Irimajiri K. Induction of apoptosis in HL-60 cells treated with medicinal herbs. Am J Chin Med. 2003;31(4):551-62 [Abstract: In order to develop a new apoptosis inducer, we screened 22 crude drugs for their apoptosis-inducing activity. It was found that Glycyrrhiza uralensis, Cynomorium songaricum, Eucommia ulmoides, Phellodendron amurense, Cinnamomum cassia and Paeonia lactiflora induced the death of HL-60 cells. To investigate the mechanism of apoptosis induced by these six crude drugs, the mitochondrial transmembrane potential and the activity of caspase-3 were measured. Reduced mitochondrial transmembrane potentials within 12 hours after the administration of Glycyrrhiza uralensis, Cynomorium songaricum, Phellodendron amurense and Paeonia lactiflora, and within 24 hours after the administration of Eucommia ulmoides and Cinnamomum cassia were observed. All of the six apoptosis-inducing crude drugs increased caspase-3 activity within 12-36 hours after administration. After further examining the apoptosis-inducing activity of berberine, palmatine, panelofuroline and glycyrrhizin, which were the ingredients obtained from Phellodendron amurense, Glycyrrhiza uralensis and Paeonia lactiflora, it was found that only berberine could induce apoptosis. From these results, it was concluded that the apoptosis induced by the six crude drugs (Glycyrrhiza uralensis, Cynomorium songaricum, Eucommia ulmoides, Phellodendron amurense, Cinnamomum cassia and Paeonia lactiflora) occurred via the mitochondrial route and that the apoptosis-conducting mechanism acted through a cascade involving caspase-3.] Kinki University School of Pharmaceutical Sciences 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.

Peng JY, Luan LJ, Chen YY. [The contents of paeoniflorin in different combination jingzhixuefuzhuyu] [Article in Chinese] Zhongguo Zhong Yao Za Zhi. 2003 Jun;28(6):516-8. [OBJECTIVE: To investigate the contents of paeoniflorin in different combination Jingzhixuefuzhuyu. METHOD: Using RP-HPLC to determine the contents of paeoniflorin in different combination Jingzhixuefuzhuyu extracts, an ODS column was used with a mobile phase of MeOH-H2O-HAC(25:75:0.15) and DAD detector at the wavelength of 230 nm. RESULT AND CONCLUSION: Different combinations of Jingzhixuefu zhuyu had great influence to the contents of paeoniflorin.] College of Pharmacy, Zhejiang University, Hangzhou 310031, Zhejiang, China.

Prieto JM, Recio MC, Giner RM, Manez S, Giner-Larza EM, Rios JL. Influence of traditional Chinese anti-inflammatory medicinal plants on leukocyte and platelet functions. J Pharm Pharmacol. 2003 Sep;55(9):1275-82. [Abstract: The enzymes 5-lipoxygenase and elastase are therapeutic targets in dermatological disorders such as psoriasis. Fifteen extracts from traditional Chinese medicinal plants used to treat topical inflammations were screened for their inhibitory effect on lipoxygenase, cyclooxygenase and elastase activity in intact leukocytes and platelets. Astragalus membranaceus, Forsythia suspensa and Poria cocos inhibited 5-lipoxygenase, with IC50 values of 141, 80 and 141 microg mL(-1), respectively. The latter two species, along with Angelica dahurica and Angelica pubescens, also inhibited elastase (IC50 values of 80, 123, 68 and 93 microg mL(-1), respectively), while A. pubescens, Atractylodes macrocephala, Lentinus edodes, Rehmannia glutinosa and Paeonia lactiflora selectively inhibited 12-(S)-HHTrE production, a valid marker of cyclooxygenase activity. The inhibition of phospholipase A(2) activity by P. cocos is discussed. Dehydrotumulosic and pachymic acids, which have been isolated from P. cocos, were shown to inhibit leukotriene B(4) release. The results indicate that both P. cocos and F. suspensa are potentially valuable species in the management of skin pathologies involving chronic inflammation.] Departament de Farmacologia, Facultat de Farmacia, Universitat de Valencia, Burjassot, Spain. Click here to read

Riaz N, Anis I, Aziz-ur-Rehman, Malik A, Ahmed Z, Muhammad P, Shujaat S, Atta-ur-Rahman. Emodinol, beta-glucuronidase inhibiting triterpene from Paeonia emodi. Nat Prod Res. 2003 Aug;17(4):247-51 [Abstract: Emodinol, a new oleane type triterpene, has been isolated from the chloroform soluble fraction of Paeonia emodi. The structure 1beta, 3beta, 23-trihydroxyolean-12-en-28-oic acid 1 has been assigned on the basis of spectral studies including 2D NMR. It showed significant beta-glucuronidase inhibitory activity. In addition benzoic acid and 3-hydroxybenzoic acid have also been reported for the first time from this species] International Centre for Chemical Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan.

Riaz N, Anis I, Malik A, Ahmed Z, Aziz-ur-rehman, Muhammad P, Nawaz SA, Choudhary MI. Paeonins A and B, lipoxygenase inhibiting monoterpene galactosides from Paeonia emodi. Chem Pharm Bull (Tokyo). 2003 Mar;51(3):252-4. [Abstract: Paeonins A and B, new monoterpene galactosides have been isolated from the chloroform-soluble fraction of the roots of Paeonia emodi and showed potent lipoxygenase inhibitory activity. The structures of 1 and 2 have been assigned on the basis of spectral analysis including one- and two-dimensional NMR techniques. ] International Centre for Chemical Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan. Click here to read

Tanaka T, Fukumori M, Ochi T, Kouno I. Paeonianins A-E, new dimeric and monomeric ellagitannins from the fruits of Paeonia lactiflora. J Nat Prod. 2003 Jun;66(6):759-63. [Abstract: Four new dimeric ellagitannins, paeonianins A-D (2-5), were isolated from the fruits of Paeonia lactiflora, together with a new ellagitannin monomer, paeonianin E (1). Their structures were determined by spectroscopic methods. Paeonianins A-D (2-5) are positional isomers formed by condensation of pentagalloyl-beta-D-glucose (8) with 5-desgalloylstachyurin (6) or casuariin (7). Paeonianin E is a C-glycosidic ellagitannin having a gallic acid methyl ester moiety at the glucose C-1 position. This is the first report of the isolation of dimeric ellagitannins from a plant in the family Paeoniaceae.] Department of Molecular Medicinal Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. Click here to read

Wang LS, Hashimoto F, Shiraishi A, Aoki N, Li JJ, Sakata Y. Chemical taxonomy of the Xibei tree peony from China by floral pigmentation. J Plant Res. 2004 Feb;117(1):47-55. Epub 2003 Dec 18. [Abstract: Petal flavonoid compositions of 39 tree peony cultivars from Xibei (northwest China) were investigated in order to study the chemotaxonomic relationship among tree peony species. Six anthocyanins, the 3- O-glucosides and 3,5-di- O-glucosides of three anthocyanidins-pelargonidin (Pg), cyanidin (Cy), and peonidin (Pn)-exist in petals without a blotch at the base. The flowers are classified into three anthocyanidin phenotypes: 'Pn, Pg>Cy'; 'Pn, Cy'; and 'Pn, Cy>Pg'. Furthermore, the yellow pigments are identified as three flavones and three flavonols: apigenin, luteolin, chrysoeriol, and kaempferol, quercetin, and isorhamnetin, respectively. Ward's minimum-variance cluster analysis with principal component analysis produced a dendrogram using standardized scores of 20 pigment variables. Of 39 cultivars, 11 clustered with white flowered Paeonia rockii and 17 with pink flowered P. rockii. The other 11 cultivars matched either P. delavayi or P. potaninii. The result suggests that the Xibei tree peony originated mainly from P. rockii.] Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. Click here to read, click here for local copy html-file .

Wu X, Wu G, Zhang W, Gu G, Sha S, Wang X. [Experiment on extraction, sulfonate of paeonol and its antibiotic effect on plant pathogen] [Article in Chinese] Zhong Yao Cai. 2003 Nov;26(11):778-80. [Abstract: Paeonol contents in root barks, cores, stems and leaves of Paeonia suffruticosa Andr. from Nanling county, Anhui province were determined and paeonol was extracted with alcohol, acid alcohol and alkali alcohol. Sodium paeonol sulfonate was synthesized by treating paeonol with concentrated sulfuric acid and oleums, and its physicochemical properties were surveyed by HPLC, TLC, FTIR and UV. Antibiotic experiment on plant pathogenic fungi and bacteria showed that paeonol and sodium paeonol sulfonate had remarkably inhibitive effects on Xanthmonas oryzae pv. oryzicola, Pseudomonas solanacearum, Phyllosticta mali and Rhizoctonia solani. Paeonol and sodium paeonol sulfonate had antibiotic effect on maize sheath blight.] College of Life Science, Nanjing Normal University, Nanjing 210097.

Yasui T, Matsuzaki T, Ushigoe K, Kuwahara A, Maegawa M, Furumoto H, Aono T, Irahara M. Stimulatory effect of the herbal medicine Keishi-bukuryo-gan on a cytokine-induced neutrophil chemoattractant, in rat ovarian cell culture. Am J Reprod Immunol. 2003 Jul;50(1):90-7.[ Abstract: We investigated the effects of Keishi-bukuryo-gan, a Japanese herbal medicine, and its crude ingredients in relation to the production of cytokine-induced neutrophil chemoattractant (CINC/gro), interleukin 1beta (IL-1beta) and tumor necrosis factor alpha (TNFalpha), which are known to stimulate the secretion of CINC/gro in the ovulatory process, and the effects of Keishi-bukuryo-gan with those of Toki-shakuyaku-san, which has been shown to have an effect on the ovary. We cultured whole ovarian dispersates from immature (3-week-old) female rats with Keishi-bukuryo-gan, Toki-shakuyaku-san and crude ingredients of Keishi-bukuryo-gan. The contents of CINC/gro, IL-1beta and TNFalpha in the cultured media were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: Keishi-bukuryo-gan stimulated the secretion of CINC/gro in a dose-dependent manner, and the secretion of CINC/gro into the culture medium increased significantly at concentrations of Keishi-bukuryo-gan of 10 and 100 microg/mL (P < 0.001). The stimulatory effect of Keishi-bukuryo-gan on the production of CINC/gro is significantly (P < 0.001) stronger than that of Toki-shakuyaku-san at the same concentrations of 100 microg/mL. In addition, Keishi-bukuryo-gan stimulated the secretion of IL-1beta in a dose-dependent manner, while it did not stimulate the secretion of TNFalpha even at a concentration of 100 microg/mL. Moutan Cortex, Paeoniae Radix and Persicae Semen, which are crude ingredients of Keishi-bukuryo-gan, enhanced the secretion of CINC/gro significantly (P < 0.01) in cultured whole ovarian dispersates. CONCLUSIONS: These results show that Keishi-bukuryo-gan can stimulate the secretion of CINC/gro as well as the production of IL-1beta and that this stimulatory effect of Keishi-bukuryo-gan was significantly stronger than that of Toki-shakuyaku-san in immature rat ovarian cell culture. PMID: 14506933 [PubMed - indexed for MEDLINE] (Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.

Yuan Tao & Wang Lianying (2003) Morphological studies on Paeonia sect. Moutan subsect. Vaginatae in China. Acta Horticulturae Sinica 30(2): 187 – 91.

Zhang GQ, Hao XM, Chen SZ, Zhou PA, Cheng HP, Wu CH. Blockade of paeoniflorin on sodium current in mouse hippocampal CA1 neurons. Acta Pharmacol Sin. 2003 Dec;24(12):1248-52. [AIM: To study the blockade of paeoniflorin (Pae) on I(Na) in the acutely isolated hippocampus neurons of mice. METHODS: The whole-cell patch clamp technique was used. RESULTS: Pae inhibited I(Na) in frequency-dependent and concentration-dependent manners, with an IC50 of 271 micromol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I(Na) inactivation state from (4.2+/-0.7) ms to (9.8+/-1.2) ms. CONCLUSION: Pae inhibited I(Na) in mouse hippocampus neurons.] State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China., Click here to read

Zhou Z Q, Pan K Y & Hong D Y, 2003: Phylogenetic analyses of Paeonia section Moutan (tree peonies, Paeoniaceae) based on morphological data. Acta Phytotax. Sin. 41 (5): 436—446. (PDF: ) (link to a local copy .pdf-file)

Carsten Burkhardt's Web Project Paeonia - The Peony Library

ano_finnland7 index 0602 boreas2

Paeonia-Literatur 2003